Add to cart Cytotoxic T cell (CTL) assay with Keytruda (anti-PD1) – IMMUNE 3D $10,000.00 Description The cytotoxic T Lymphocyte (CTL) response is recognized as perhaps the most relevant functional measure that reflects cell-mediated acquired immune defense against viral infections and cancer. The CTL responses can evaluate effective antigen presentation by tumors and is a key assessment in how well a drug candidate enhances CTL responses in eradicating or killing tumors. IMMUNE 3D® is a physiologically relevant platform that is designed to capture the immune-tumor interactions in the presence of tumor environment cells. This assay utilizes our proprietary HLA-matched CTL and tumor cell lines and is run in a 24 well format. What is the assay setup? Co-culture tumor and CTL for 6 days in the presence of drug candidate After 6 days harvest the supernatants Measure cytokine release and tumor lysis What are the study arms? CTL + Tumor+ Tecentriq (anti-PDL1) CTL + Tumor+ <drug candidate (per dose)> CTL + Tumor CTL + Tumor + DMSO CTL Tumor How many doses are included? The assay is run in a 24 well plate and can accommodate up to 7 doses of the drug candidate. Each dose is a well. Each dose is run in duplicates (2 times) What readouts are included in the price? Multiplex cytokine readouts – IL-2, IL-6, IL-4, IL-5, IL-9 IL-10, IL-13,IL-17, TNF-α, IFN-γ Lactate dehyrdogenase (LDH) for tumor lysis Assay setup and methods used Raw data files for cytokines When can I expect results? We project results are available in 2-3 weeks. We can accelerate results on a case-by-case basis. Contact us and we can work with you to accommodate your requests.